Application

Detect Cereblon using this mouse monoclonal Anti-Cereblon Antibody, clone 3B12F7, Cat. No. MABN1780, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected cereblon in 10 µg of HEK293 cell lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected cereblon in human crebral cortex, kidney, and tonsil tissue sections.

General description

Protein cereblon (UniProt Q96SW2) is encoded by the CRBN (also known as AD-006, MRT2A) gene (Gene ID 51185) in human. Originally identified as a genetic cause of inherited autosomal recessive mental retardation, the human CRBN gene encodes a substrate receptor of a ubiquitin E3 cullin 4-RING ligase (CRL4) complex. A cullin-4 (CULT4) protein provides a scaffold for assembling RING box-domain protein (RBX1) and the adaptor protein damaged DNA-binding protein 1 (DDB1) to form a CRL4 E3 complex, where RBX1 serves as the docking site for the activated E2 protein, and DDB1 recruits substrate-specificity receptors or DCAFs (DDB1-cullin 4-associated factors) to form the substrate-presenting side of the CRL4 complex. Thalidomide and its analogs (lenalidomide, pomalidomide and apremilast) are immunomodulatory drugs (IMiDs) that exert their antiproliferative effects on multiple myeloma (MM) cells and immunomodulatory effects on T cells by directly binding cereblon and promoting recruitment of the hematopoietic zinc-finger transcription factors Ikaros (IKZF1) and Aiolos (IKZF3), leading to their ubiquitination and proteasomal degradation. Human cereblon is a 442-amino acid protein composed of a Lon protease-like scaffold (a.a. 80-317) with a helical insertion that mediates DDB1 binding and a thalidomide-binding domain (TBD; a.a. 339-442). r>

Immunogen

KLH-conjugated linear peptide corresponding to a sequence near the N-terminus of human cereblon.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected cereblon in 10 µg of Jurkat cell lysate.

Specificity

Clone 3B12F7 was raised against an N-terminal sequence present in human cereblon spliced isoforms 1&2 and murine cereblon spliced isoforms 1&3 (UniProt Q96SW2 and Q8C7D2). Immunogen sequence is well conserved in essentially all mammalian species.

Target description

~50 kDa observed. 50.55/50.48 kDa (human isoform 1/2) and 50.88/50.81 kDa (mouse isoform 1/3) calculated. Uncharacterized bands may be observed in some lysate(s).